AmScope FM580BA Binocular Compound Epi-Fluorescence Microscope, WF10x and WF16x Eyepieces, 40X-1600X Magnification, Brightfield, 30W Halogen Illumination, Abbe Condenser, Double-Layer Mechanical Stage, Anti-Mold, 110V, Includes 4 Fluorescence Objectives
- Epi-fluorescence microscope with mercury lamp light source for performing sample analysis of biological specimens, sedimentary rock, and semiconductor impurities
- Siedentopf binocular viewing head with interchangeable pairs of 10x widefield and 16x widefield eyepieces, a sliding head with 50 to 75mm interpupillary adjustment, and a fixed 30-degree vertical inclination to reduce eye and neck strain
- Forward-facing revolving quadruple nosepiece with 4x, 10x, 40xS (spring), and 100xS (spring, oil) DIN fluorescence infinity plan objectives
- 30W halogen and 100W HBO ultra-high voltage spherical mercury lamp with 1.25 NA Abbe condenser on a rack-and-pinion focus system and iris diaphragm
- Double-layer mechanical stage with 1mm stage divisions and stage stop; graduated coarse and fine focus with tension control to prevent stage drift
Publisher: United Scope LLC.
The AmScope FM580BA epi-fluorescence binocular compound microscope has interchangeable pairs of 10x widefield and 16x widefield eyepieces, a forward-facing nosepiece with four DIN infinity plan fluorescence objectives, Brightfield and fluorescence illumination, a double-layer mechanical stage with a stage stop to protect slides and objectives from damage, and anti-mold surfaces to preserve optics in high-humidity areas. The Siedentopf binocular head has 50 to 75mm interpupillary adjustment, interchangeable pairs of WF10x and WF16x eyepieces, and a fixed 30-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to re-focus the image. Dioptric adjustment accommodates individual eye-strength differences. The forward-facing revolving nosepiece accepts up to four 20mm objectives. Four DIN infinity plan fluorescence objectives (4x, 10x, and 40xS, and 100xS) are designed for use with fluorescence illumination to provide improved focus over the entire viewing field. Infinity plan objectives provide focus over the entire range of the viewing field, and a longer working distance for improved focus when auxiliary components are utilized. The 40xS objectives are spring loaded to prevent slide damage when focusing. The 100xS spring-loaded oil immersion objective uses oil between the specimen and the objective lens to provide increased resolution. Compound fluorescence microscopes are used in biological and metallurgical research and in industrial inspection applications to observe details illuminated by ultraviolet light.
The microscope has upper (incidental, episcopic) fluorescence illumination that reflects light off the specimen for enhanced visibility of opaque specimens, and lower (transmitted, diascopic) Brightfield illumination that transmits light up through the specimen for enhanced visibility of translucent and transparent objects. Brightfield (BF) illumination allows the specimen to absorb light, resulting in a dark image on a light background. Fluorescence illumination uses a mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. An epi-fluorescence microscope, or incident-light fluorescence, has the lamp and excitation filter mounted above the objective. Transmitted 30W halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. The 1.25 NA Abbe condenser is mounted on a rack-and-pinion control system, can be adjusted to control the distance of the light from the stage, has a swing-out color filter holder, and has an iris diaphragm to optimize the amount of light illuminating the specimen. The double-layer mechanical stage, with 1mm stage divisions, locks the slide into place and provides precise slide manipulation along the X- and Y-axis to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. A stage stop prevents the stage from coming into contact with the slide and objectives. The stage is 7.10 x 5.90 inches (180 x 150mm) and has a traveling range of 2.95 x 1.97 inches (75 x 50mm). Graduated coaxial coarse and fine focus has fine-focus divisions of 0.002mm (0.0000787") that enable measurements to be taken. Focus knob tension control prevents the stage from drifting out of focus. The cast-alloy enamel-coated body is durable and resistant to stains and corrosion.
|Eyepieces (23mm)||WF10x, WF16x|
|Fluorescence objectives (20mm)||4X, 10x, 40xS, 100xS (oil)|
|Lighting configuration||Transmitted (lower) and incidental (upper)|
|Condenser||1.25 NA Abbe|
|Light source||30W Halogen with rheostat (transmitted), and mercury lamp (incidental)|
|Illumination type||Fluorescence and Brightfield|
Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.
United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.
What's in the Box?
- AmScope FM580BA microscope with double-layer mechanical stage
- WF10x eyepieces, 23mm, one pair
- WF16x eyepieces, 23mm, one pair
- 4x DIN fluorescence plan objective, 20mm
- 10x DIN fluorescence plan objective, 20mm
- 40xS DIN fluorescence plan objective, 20mm
- 100xS (oil) DIN fluorescence plan objective, 20mm
- Fluorescence head channel and filter selector
- Power box
- Mercury light housing box
- Fluorescence shield
- (2) Color filters; blue and green
- Spare halogen bulb
- Spare fuse
- Immersion oil, one bottle